A3243G
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Cybrid Cells
Cybrid Cells are used to investigate the effects of mtDNA mutations.
Firstly a cultured human cell has it's Mitochondria removed; then a cell from a heteroplasmic individual
has it nucleus removed; finally these two cells are merged.
Suppose a heteroplasmic individual has a mutation load of 60%: The resulting
Cybrid Cell will also have a mutation load of 60%.
This Cybrid Cell is then grown many times. As the Cybrid Cells divide,
(Mitosis), the mitochondria are randomly divided between the two new cells.
Thus, the daughter cells may have a higher or lower mutation load. If these cells are selected
and grown, strains of Cybrid Cells can be grown which have higher or lower
mutation loads.
Once this process of selection and re-growing has been repeated several times,
batches of Cybrid Cells can be created which have average mutation loads of 70%, 80%, even 90%
mutation load.
The behaviour of these cells can then be examined with constant Nuclear DNA
input - the only difference being the heteroplasmic mutation load.
See Also
mtDNA
Heteroplasmy
Mitochondria
Mitosis
Author: Andy Collinson.
Although I don't have any medical qualifications,
as a sufferer of Diabetes, Deafness and Tinnitus caused by the A3243G mtDNA defect,
I do have a very keen interest in the subject.
Date Page Updated:
25 April 2005
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